Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
134 |
6.0% |
| Arg[R] |
110 |
4.9% |
| Asn[N] |
145 |
6.5% |
| Asp[D] |
133 |
6.0% |
| Cys[C] |
31 |
1.4% |
| Gln[Q] |
84 |
3.8% |
| Glu[E] |
138 |
6.2% |
| Gly[G] |
87 |
3.9% |
| His[H] |
42 |
1.9% |
| Ile[I] |
146 |
6.5% |
| Leu[L] |
211 |
9.5% |
| Lys[K] |
188 |
8.4% |
| Met[M] |
42 |
1.9% |
| Phe[F] |
102 |
4.6% |
| Pro[P] |
108 |
4.8% |
| Ser[S] |
210 |
9.4% |
| Thr[T] |
110 |
4.9% |
| Trp[W] |
19 |
0.9% |
| Tyr[Y] |
63 |
2.8% |
| Val[V] |
128 |
5.7% |
| Total Length |
2231 |
|
| Instability index (II) |
43.89 |
| This classifies the protein as unstable |
|
| Carbon |
11227 |
| Hydrogen |
17839 |
| Nitrogen |
3081 |
| Oxygen |
3386 |
| Sulfur |
73 |
| Total number of atoms |
35606 |
| Formula = C11227H17839N3081O3386S73 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
200245 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
198370 M-1 cm-1 |
| assuming all Cys residues are reduced |
198370 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
200245 M-1 cm-1 |
|
|
| Codon Bias |
0.072 |
| Codon Adaptation Index |
0.150 |
| Frequency of Optimal Codons |
0.451 |
| Hydropathicity of Protein |
-0.441 |
| Aromaticity Score |
0.082 |
|
