Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
62 |
4.7% |
| Arg[R] |
57 |
4.4% |
| Asn[N] |
82 |
6.3% |
| Asp[D] |
78 |
6.0% |
| Cys[C] |
19 |
1.5% |
| Gln[Q] |
42 |
3.2% |
| Glu[E] |
87 |
6.7% |
| Gly[G] |
49 |
3.7% |
| His[H] |
26 |
2.0% |
| Ile[I] |
92 |
7.0% |
| Leu[L] |
117 |
9.0% |
| Lys[K] |
100 |
7.7% |
| Met[M] |
20 |
1.5% |
| Phe[F] |
78 |
6.0% |
| Pro[P] |
68 |
5.2% |
| Ser[S] |
127 |
9.7% |
| Thr[T] |
73 |
5.6% |
| Trp[W] |
13 |
1.0% |
| Tyr[Y] |
45 |
3.4% |
| Val[V] |
72 |
5.5% |
| Total Length |
1307 |
|
| Instability index (II) |
46.25 |
| This classifies the protein as unstable |
|
| Carbon |
6701 |
| Hydrogen |
10433 |
| Nitrogen |
1767 |
| Oxygen |
2007 |
| Sulfur |
39 |
| Total number of atoms |
20947 |
| Formula = C6701H10433N1767O2007S39 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
139675 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
138550 M-1 cm-1 |
| assuming all Cys residues are reduced |
138550 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
139675 M-1 cm-1 |
|
|
| Codon Bias |
-0.003 |
| Codon Adaptation Index |
0.125 |
| Frequency of Optimal Codons |
0.413 |
| Hydropathicity of Protein |
-0.395 |
| Aromaticity Score |
0.104 |
|
