Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
22 |
3.2% |
| Arg[R] |
25 |
3.6% |
| Asn[N] |
46 |
6.7% |
| Asp[D] |
45 |
6.5% |
| Cys[C] |
7 |
1.0% |
| Gln[Q] |
35 |
5.1% |
| Glu[E] |
49 |
7.1% |
| Gly[G] |
29 |
4.2% |
| His[H] |
15 |
2.2% |
| Ile[I] |
57 |
8.2% |
| Leu[L] |
91 |
13.2% |
| Lys[K] |
44 |
6.4% |
| Met[M] |
14 |
2.0% |
| Phe[F] |
29 |
4.2% |
| Pro[P] |
21 |
3.0% |
| Ser[S] |
56 |
8.1% |
| Thr[T] |
46 |
6.7% |
| Trp[W] |
8 |
1.2% |
| Tyr[Y] |
22 |
3.2% |
| Val[V] |
30 |
4.3% |
| Total Length |
691 |
|
| Instability index (II) |
42.23 |
| This classifies the protein as unstable |
|
| Carbon |
3545 |
| Hydrogen |
5603 |
| Nitrogen |
929 |
| Oxygen |
1085 |
| Sulfur |
21 |
| Total number of atoms |
11183 |
| Formula = C3545H5603N929O1085S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
77155 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
76780 M-1 cm-1 |
| assuming all Cys residues are reduced |
76780 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
77155 M-1 cm-1 |
|
|
| Codon Bias |
-0.010 |
| Codon Adaptation Index |
0.120 |
| Frequency of Optimal Codons |
0.410 |
| Hydropathicity of Protein |
-0.303 |
| Aromaticity Score |
0.085 |
|
