Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
64 |
5.7% |
| Arg[R] |
76 |
6.8% |
| Asn[N] |
75 |
6.7% |
| Asp[D] |
72 |
6.5% |
| Cys[C] |
11 |
1.0% |
| Gln[Q] |
67 |
6.0% |
| Glu[E] |
105 |
9.4% |
| Gly[G] |
47 |
4.2% |
| His[H] |
14 |
1.3% |
| Ile[I] |
86 |
7.7% |
| Leu[L] |
99 |
8.9% |
| Lys[K] |
101 |
9.1% |
| Met[M] |
23 |
2.1% |
| Phe[F] |
36 |
3.2% |
| Pro[P] |
18 |
1.6% |
| Ser[S] |
88 |
7.9% |
| Thr[T] |
52 |
4.7% |
| Trp[W] |
3 |
0.3% |
| Tyr[Y] |
28 |
2.5% |
| Val[V] |
49 |
4.4% |
| Total Length |
1114 |
|
| Instability index (II) |
40.76 |
| This classifies the protein as unstable |
|
| Carbon |
5554 |
| Hydrogen |
9040 |
| Nitrogen |
1616 |
| Oxygen |
1779 |
| Sulfur |
34 |
| Total number of atoms |
18023 |
| Formula = C5554H9040N1616O1779S34 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
58845 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
58220 M-1 cm-1 |
| assuming all Cys residues are reduced |
58220 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
58845 M-1 cm-1 |
|
|
| Codon Bias |
0.061 |
| Codon Adaptation Index |
0.151 |
| Frequency of Optimal Codons |
0.452 |
| Hydropathicity of Protein |
-0.749 |
| Aromaticity Score |
0.060 |
|
