FKS1/YLR342W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 138 7.4%
Arg[R] 102 5.4%
Asn[N] 77 4.1%
Asp[D] 89 4.7%
Cys[C] 33 1.8%
Gln[Q] 80 4.3%
Glu[E] 100 5.3%
Gly[G] 118 6.3%
His[H] 43 2.3%
Ile[I] 134 7.1%
Leu[L] 175 9.3%
Lys[K] 82 4.4%
Met[M] 55 2.9%
Phe[F] 115 6.1%
Pro[P] 86 4.6%
Ser[S] 114 6.1%
Thr[T] 102 5.4%
Trp[W] 37 2.0%
Tyr[Y] 99 5.3%
Val[V] 97 5.2%
Total Length 1876

Instability index
Instability index (II) 40.44
This classifies the protein as unstable

Atomic composition
Carbon 9802
Hydrogen 14916
Nitrogen 2544
Oxygen 2727
Sulfur 88
Total number of atoms 30077
Formula = C9802H14916N2544O2727S88

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 353010 M-1 cm-1
assuming NO Cys residues appear as half cystines 351010 M-1 cm-1
assuming all Cys residues are reduced 351010 M-1 cm-1
assuming all pairs of Cys residues form cystines 353010 M-1 cm-1

Aliphatic index
Aliphatic index 86.59

Coding Region Translation Calculations
Codon Bias 0.301
Codon Adaptation Index 0.273
Frequency of Optimal Codons 0.582
Hydropathicity of Protein -0.109
Aromaticity Score 0.134