VRP1/YLR337C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 77 9.4%
Arg[R] 19 2.3%
Asn[N] 35 4.3%
Asp[D] 28 3.4%
Cys[C] 0 0.0%
Gln[Q] 21 2.6%
Glu[E] 15 1.8%
Gly[G] 45 5.5%
His[H] 15 1.8%
Ile[I] 33 4.0%
Leu[L] 49 6.0%
Lys[K] 47 5.8%
Met[M] 13 1.6%
Phe[F] 11 1.3%
Pro[P] 183 22.4%
Ser[S] 126 15.4%
Thr[T] 52 6.4%
Trp[W] 1 0.1%
Tyr[Y] 3 0.4%
Val[V] 44 5.4%
Total Length 817

Instability index
Instability index (II) 80.36
This classifies the protein as unstable

Atomic composition
Carbon 3654
Hydrogen 5868
Nitrogen 1008
Oxygen 1141
Sulfur 13
Total number of atoms 11684
Formula = C3654H5868N1008O1141S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 9970 M-1 cm-1

Aliphatic index
Aliphatic index 64.19

Coding Region Translation Calculations
Codon Bias 0.075
Codon Adaptation Index 0.109
Frequency of Optimal Codons 0.406
Hydropathicity of Protein -0.493
Aromaticity Score 0.018