Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
59 |
8.2% |
| Arg[R] |
15 |
2.1% |
| Asn[N] |
51 |
7.1% |
| Asp[D] |
50 |
6.9% |
| Cys[C] |
1 |
0.1% |
| Gln[Q] |
18 |
2.5% |
| Glu[E] |
54 |
7.5% |
| Gly[G] |
33 |
4.6% |
| His[H] |
1 |
0.1% |
| Ile[I] |
19 |
2.6% |
| Leu[L] |
32 |
4.4% |
| Lys[K] |
89 |
12.4% |
| Met[M] |
8 |
1.1% |
| Phe[F] |
45 |
6.2% |
| Pro[P] |
48 |
6.7% |
| Ser[S] |
99 |
13.8% |
| Thr[T] |
60 |
8.3% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
6 |
0.8% |
| Val[V] |
32 |
4.4% |
| Total Length |
720 |
|
| Instability index (II) |
38.60 |
| This classifies the protein as stable |
|
| Carbon |
3382 |
| Hydrogen |
5365 |
| Nitrogen |
925 |
| Oxygen |
1163 |
| Sulfur |
9 |
| Total number of atoms |
10844 |
| Formula = C3382H5365N925O1163S9 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
8940 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
8940 M-1 cm-1 |
| assuming all Cys residues are reduced |
8940 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
8940 M-1 cm-1 |
|
|
| Codon Bias |
0.050 |
| Codon Adaptation Index |
0.143 |
| Frequency of Optimal Codons |
0.440 |
| Hydropathicity of Protein |
-0.904 |
| Aromaticity Score |
0.071 |
|
