RPS25B/YLR333C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 15 13.9%
Arg[R] 7 6.5%
Asn[N] 0 0.0%
Asp[D] 4 3.7%
Cys[C] 0 0.0%
Gln[Q] 5 4.6%
Glu[E] 5 4.6%
Gly[G] 5 4.6%
His[H] 3 2.8%
Ile[I] 8 7.4%
Leu[L] 9 8.3%
Lys[K] 18 16.7%
Met[M] 2 1.9%
Phe[F] 0 0.0%
Pro[P] 4 3.7%
Ser[S] 10 9.3%
Thr[T] 2 1.9%
Trp[W] 1 0.9%
Tyr[Y] 4 3.7%
Val[V] 6 5.6%
Total Length 108

Instability index
Instability index (II) 26.94
This classifies the protein as stable

Atomic composition
Carbon 536
Hydrogen 905
Nitrogen 159
Oxygen 148
Sulfur 2
Total number of atoms 1750
Formula = C536H905N159O148S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 11460 M-1 cm-1

Aliphatic index
Aliphatic index 91.39

Coding Region Translation Calculations
Codon Bias 0.704
Codon Adaptation Index 0.612
Frequency of Optimal Codons 0.819
Hydropathicity of Protein -0.537
Aromaticity Score 0.046