Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
47 |
7.0% |
| Arg[R] |
14 |
2.1% |
| Asn[N] |
49 |
7.3% |
| Asp[D] |
42 |
6.3% |
| Cys[C] |
5 |
0.7% |
| Gln[Q] |
20 |
3.0% |
| Glu[E] |
97 |
14.5% |
| Gly[G] |
24 |
3.6% |
| His[H] |
14 |
2.1% |
| Ile[I] |
30 |
4.5% |
| Leu[L] |
39 |
5.8% |
| Lys[K] |
55 |
8.2% |
| Met[M] |
7 |
1.0% |
| Phe[F] |
10 |
1.5% |
| Pro[P] |
44 |
6.6% |
| Ser[S] |
61 |
9.1% |
| Thr[T] |
49 |
7.3% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
6 |
0.9% |
| Val[V] |
55 |
8.2% |
| Total Length |
671 |
|
| Instability index (II) |
60.51 |
| This classifies the protein as unstable |
|
| Carbon |
3151 |
| Hydrogen |
5050 |
| Nitrogen |
868 |
| Oxygen |
1135 |
| Sulfur |
12 |
| Total number of atoms |
10216 |
| Formula = C3151H5050N868O1135S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
25690 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
25440 M-1 cm-1 |
| assuming all Cys residues are reduced |
25440 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
25690 M-1 cm-1 |
|
|
| Codon Bias |
0.071 |
| Codon Adaptation Index |
0.152 |
| Frequency of Optimal Codons |
0.463 |
| Hydropathicity of Protein |
-0.851 |
| Aromaticity Score |
0.028 |
|
