EST2/YLR318W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 32 3.6%
Arg[R] 36 4.1%
Asn[N] 68 7.7%
Asp[D] 39 4.4%
Cys[C] 18 2.0%
Gln[Q] 37 4.2%
Glu[E] 40 4.5%
Gly[G] 23 2.6%
His[H] 22 2.5%
Ile[I] 83 9.4%
Leu[L] 100 11.3%
Lys[K] 75 8.5%
Met[M] 14 1.6%
Phe[F] 59 6.7%
Pro[P] 32 3.6%
Ser[S] 69 7.8%
Thr[T] 49 5.5%
Trp[W] 8 0.9%
Tyr[Y] 32 3.6%
Val[V] 48 5.4%
Total Length 884

Instability index
Instability index (II) 38.63
This classifies the protein as stable

Atomic composition
Carbon 4685
Hydrogen 7368
Nitrogen 1224
Oxygen 1298
Sulfur 32
Total number of atoms 14607
Formula = C4685H7368N1224O1298S32

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 92805 M-1 cm-1
assuming NO Cys residues appear as half cystines 91680 M-1 cm-1
assuming all Cys residues are reduced 91680 M-1 cm-1
assuming all pairs of Cys residues form cystines 92805 M-1 cm-1

Aliphatic index
Aliphatic index 100.10

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000