Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
41 |
4.5% |
| Arg[R] |
32 |
3.5% |
| Asn[N] |
75 |
8.2% |
| Asp[D] |
54 |
5.9% |
| Cys[C] |
3 |
0.3% |
| Gln[Q] |
43 |
4.7% |
| Glu[E] |
130 |
14.3% |
| Gly[G] |
16 |
1.8% |
| His[H] |
11 |
1.2% |
| Ile[I] |
44 |
4.8% |
| Leu[L] |
119 |
13.1% |
| Lys[K] |
132 |
14.5% |
| Met[M] |
10 |
1.1% |
| Phe[F] |
9 |
1.0% |
| Pro[P] |
11 |
1.2% |
| Ser[S] |
83 |
9.1% |
| Thr[T] |
48 |
5.3% |
| Trp[W] |
2 |
0.2% |
| Tyr[Y] |
19 |
2.1% |
| Val[V] |
29 |
3.2% |
| Total Length |
911 |
|
| Instability index (II) |
50.48 |
| This classifies the protein as unstable |
|
| Carbon |
4538 |
| Hydrogen |
7533 |
| Nitrogen |
1281 |
| Oxygen |
1548 |
| Sulfur |
13 |
| Total number of atoms |
14913 |
| Formula = C4538H7533N1281O1548S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
39435 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
39310 M-1 cm-1 |
| assuming all Cys residues are reduced |
39310 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
39435 M-1 cm-1 |
|
|
| Codon Bias |
0.048 |
| Codon Adaptation Index |
0.153 |
| Frequency of Optimal Codons |
0.446 |
| Hydropathicity of Protein |
-1.102 |
| Aromaticity Score |
0.033 |
|
