Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
121 |
6.4% |
| Arg[R] |
75 |
3.9% |
| Asn[N] |
90 |
4.7% |
| Asp[D] |
99 |
5.2% |
| Cys[C] |
28 |
1.5% |
| Gln[Q] |
66 |
3.5% |
| Glu[E] |
104 |
5.5% |
| Gly[G] |
80 |
4.2% |
| His[H] |
44 |
2.3% |
| Ile[I] |
147 |
7.7% |
| Leu[L] |
227 |
11.9% |
| Lys[K] |
127 |
6.7% |
| Met[M] |
35 |
1.8% |
| Phe[F] |
107 |
5.6% |
| Pro[P] |
71 |
3.7% |
| Ser[S] |
177 |
9.3% |
| Thr[T] |
123 |
6.5% |
| Trp[W] |
16 |
0.8% |
| Tyr[Y] |
69 |
3.6% |
| Val[V] |
94 |
4.9% |
| Total Length |
1900 |
|
| Instability index (II) |
37.11 |
| This classifies the protein as stable |
|
| Carbon |
9716 |
| Hydrogen |
15272 |
| Nitrogen |
2512 |
| Oxygen |
2832 |
| Sulfur |
63 |
| Total number of atoms |
30395 |
| Formula = C9716H15272N2512O2832S63 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
192560 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
190810 M-1 cm-1 |
| assuming all Cys residues are reduced |
190810 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
192560 M-1 cm-1 |
|
|
| Codon Bias |
0.072 |
| Codon Adaptation Index |
0.144 |
| Frequency of Optimal Codons |
0.449 |
| Hydropathicity of Protein |
-0.071 |
| Aromaticity Score |
0.101 |
|
