Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
67 |
8.6% |
| Arg[R] |
33 |
4.2% |
| Asn[N] |
42 |
5.4% |
| Asp[D] |
57 |
7.3% |
| Cys[C] |
7 |
0.9% |
| Gln[Q] |
22 |
2.8% |
| Glu[E] |
37 |
4.8% |
| Gly[G] |
70 |
9.0% |
| His[H] |
17 |
2.2% |
| Ile[I] |
52 |
6.7% |
| Leu[L] |
61 |
7.8% |
| Lys[K] |
64 |
8.2% |
| Met[M] |
16 |
2.1% |
| Phe[F] |
28 |
3.6% |
| Pro[P] |
42 |
5.4% |
| Ser[S] |
37 |
4.8% |
| Thr[T] |
47 |
6.0% |
| Trp[W] |
10 |
1.3% |
| Tyr[Y] |
23 |
3.0% |
| Val[V] |
46 |
5.9% |
| Total Length |
778 |
|
| Instability index (II) |
25.73 |
| This classifies the protein as stable |
|
| Carbon |
3803 |
| Hydrogen |
6005 |
| Nitrogen |
1049 |
| Oxygen |
1138 |
| Sulfur |
23 |
| Total number of atoms |
12018 |
| Formula = C3803H6005N1049O1138S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
89645 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
89270 M-1 cm-1 |
| assuming all Cys residues are reduced |
89270 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
89645 M-1 cm-1 |
|
|
| Codon Bias |
0.513 |
| Codon Adaptation Index |
0.462 |
| Frequency of Optimal Codons |
0.709 |
| Hydropathicity of Protein |
-0.381 |
| Aromaticity Score |
0.078 |
|
