Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
8.8% |
| Arg[R] |
12 |
2.7% |
| Asn[N] |
22 |
5.0% |
| Asp[D] |
21 |
4.7% |
| Cys[C] |
1 |
0.2% |
| Gln[Q] |
18 |
4.1% |
| Glu[E] |
27 |
6.1% |
| Gly[G] |
42 |
9.5% |
| His[H] |
16 |
3.6% |
| Ile[I] |
24 |
5.4% |
| Leu[L] |
35 |
7.9% |
| Lys[K] |
27 |
6.1% |
| Met[M] |
2 |
0.5% |
| Phe[F] |
26 |
5.9% |
| Pro[P] |
22 |
5.0% |
| Ser[S] |
29 |
6.5% |
| Thr[T] |
24 |
5.4% |
| Trp[W] |
4 |
0.9% |
| Tyr[Y] |
20 |
4.5% |
| Val[V] |
33 |
7.4% |
| Total Length |
444 |
|
| Instability index (II) |
23.11 |
| This classifies the protein as stable |
|
| Carbon |
2211 |
| Hydrogen |
3363 |
| Nitrogen |
583 |
| Oxygen |
654 |
| Sulfur |
3 |
| Total number of atoms |
6814 |
| Formula = C2211H3363N583O654S3 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
51800 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
51800 M-1 cm-1 |
| assuming all Cys residues are reduced |
51800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
51800 M-1 cm-1 |
|
|
| Codon Bias |
0.533 |
| Codon Adaptation Index |
0.460 |
| Frequency of Optimal Codons |
0.721 |
| Hydropathicity of Protein |
-0.250 |
| Aromaticity Score |
0.113 |
|
