MEC3/YLR288C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 18 3.8%
Arg[R] 26 5.5%
Asn[N] 31 6.5%
Asp[D] 31 6.5%
Cys[C] 10 2.1%
Gln[Q] 13 2.7%
Glu[E] 37 7.8%
Gly[G] 32 6.8%
His[H] 13 2.7%
Ile[I] 39 8.2%
Leu[L] 39 8.2%
Lys[K] 23 4.9%
Met[M] 14 3.0%
Phe[F] 12 2.5%
Pro[P] 17 3.6%
Ser[S] 41 8.6%
Thr[T] 33 7.0%
Trp[W] 5 1.1%
Tyr[Y] 12 2.5%
Val[V] 28 5.9%
Total Length 474

Instability index
Instability index (II) 36.44
This classifies the protein as stable

Atomic composition
Carbon 2307
Hydrogen 3678
Nitrogen 650
Oxygen 741
Sulfur 24
Total number of atoms 7400
Formula = C2307H3678N650O741S24

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 46005 M-1 cm-1
assuming NO Cys residues appear as half cystines 45380 M-1 cm-1
assuming all Cys residues are reduced 45380 M-1 cm-1
assuming all pairs of Cys residues form cystines 46005 M-1 cm-1

Aliphatic index
Aliphatic index 85.11

Coding Region Translation Calculations
Codon Bias 0.005
Codon Adaptation Index 0.108
Frequency of Optimal Codons 0.418
Hydropathicity of Protein -0.416
Aromaticity Score 0.061