RPS30A/YLR287C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 4 6.3%
Arg[R] 7 11.1%
Asn[N] 3 4.8%
Asp[D] 0 0.0%
Cys[C] 0 0.0%
Gln[Q] 2 3.2%
Glu[E] 2 3.2%
Gly[G] 5 7.9%
His[H] 1 1.6%
Ile[I] 0 0.0%
Leu[L] 4 6.3%
Lys[K] 11 17.5%
Met[M] 2 3.2%
Phe[F] 1 1.6%
Pro[P] 5 7.9%
Ser[S] 3 4.8%
Thr[T] 4 6.3%
Trp[W] 0 0.0%
Tyr[Y] 2 3.2%
Val[V] 7 11.1%
Total Length 63

Instability index
Instability index (II) 31.38
This classifies the protein as stable

Atomic composition
Carbon 314
Hydrogen 538
Nitrogen 102
Oxygen 82
Sulfur 2
Total number of atoms 1038
Formula = C314H538N102O82S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 63.33

Coding Region Translation Calculations
Codon Bias 0.712
Codon Adaptation Index 0.642
Frequency of Optimal Codons 0.820
Hydropathicity of Protein -0.976
Aromaticity Score 0.048