Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
58 |
10.3% |
| Arg[R] |
4 |
0.7% |
| Asn[N] |
23 |
4.1% |
| Asp[D] |
27 |
4.8% |
| Cys[C] |
12 |
2.1% |
| Gln[Q] |
25 |
4.4% |
| Glu[E] |
21 |
3.7% |
| Gly[G] |
32 |
5.7% |
| His[H] |
2 |
0.4% |
| Ile[I] |
23 |
4.1% |
| Leu[L] |
51 |
9.1% |
| Lys[K] |
22 |
3.9% |
| Met[M] |
3 |
0.5% |
| Phe[F] |
25 |
4.4% |
| Pro[P] |
14 |
2.5% |
| Ser[S] |
94 |
16.7% |
| Thr[T] |
81 |
14.4% |
| Trp[W] |
6 |
1.1% |
| Tyr[Y] |
20 |
3.6% |
| Val[V] |
19 |
3.4% |
| Total Length |
562 |
|
| Instability index (II) |
37.70 |
| This classifies the protein as stable |
|
| Carbon |
2573 |
| Hydrogen |
4006 |
| Nitrogen |
654 |
| Oxygen |
902 |
| Sulfur |
15 |
| Total number of atoms |
8150 |
| Formula = C2573H4006N654O902S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
63550 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
62800 M-1 cm-1 |
| assuming all Cys residues are reduced |
62800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
63550 M-1 cm-1 |
|
|
| Codon Bias |
0.218 |
| Codon Adaptation Index |
0.210 |
| Frequency of Optimal Codons |
0.530 |
| Hydropathicity of Protein |
-0.102 |
| Aromaticity Score |
0.091 |
|
