Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
4.5% |
| Arg[R] |
26 |
4.4% |
| Asn[N] |
31 |
5.2% |
| Asp[D] |
34 |
5.7% |
| Cys[C] |
5 |
0.8% |
| Gln[Q] |
32 |
5.4% |
| Glu[E] |
45 |
7.6% |
| Gly[G] |
29 |
4.9% |
| His[H] |
13 |
2.2% |
| Ile[I] |
38 |
6.4% |
| Leu[L] |
69 |
11.6% |
| Lys[K] |
70 |
11.8% |
| Met[M] |
7 |
1.2% |
| Phe[F] |
25 |
4.2% |
| Pro[P] |
22 |
3.7% |
| Ser[S] |
33 |
5.6% |
| Thr[T] |
28 |
4.7% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
20 |
3.4% |
| Val[V] |
40 |
6.7% |
| Total Length |
594 |
|
| Instability index (II) |
39.77 |
| This classifies the protein as stable |
|
| Carbon |
3056 |
| Hydrogen |
4939 |
| Nitrogen |
831 |
| Oxygen |
897 |
| Sulfur |
12 |
| Total number of atoms |
9735 |
| Formula = C3056H4939N831O897S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
30050 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
29800 M-1 cm-1 |
| assuming all Cys residues are reduced |
29800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
30050 M-1 cm-1 |
|
|
| Codon Bias |
0.116 |
| Codon Adaptation Index |
0.180 |
| Frequency of Optimal Codons |
0.482 |
| Hydropathicity of Protein |
-0.508 |
| Aromaticity Score |
0.076 |
|
