Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
58 |
4.9% |
| Arg[R] |
40 |
3.4% |
| Asn[N] |
71 |
6.0% |
| Asp[D] |
60 |
5.1% |
| Cys[C] |
23 |
2.0% |
| Gln[Q] |
55 |
4.7% |
| Glu[E] |
98 |
8.3% |
| Gly[G] |
46 |
3.9% |
| His[H] |
17 |
1.4% |
| Ile[I] |
87 |
7.4% |
| Leu[L] |
162 |
13.8% |
| Lys[K] |
93 |
7.9% |
| Met[M] |
31 |
2.6% |
| Phe[F] |
47 |
4.0% |
| Pro[P] |
28 |
2.4% |
| Ser[S] |
109 |
9.3% |
| Thr[T] |
60 |
5.1% |
| Trp[W] |
5 |
0.4% |
| Tyr[Y] |
27 |
2.3% |
| Val[V] |
59 |
5.0% |
| Total Length |
1176 |
|
| Instability index (II) |
41.21 |
| This classifies the protein as unstable |
|
| Carbon |
5896 |
| Hydrogen |
9538 |
| Nitrogen |
1554 |
| Oxygen |
1815 |
| Sulfur |
54 |
| Total number of atoms |
18857 |
| Formula = C5896H9538N1554O1815S54 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
69105 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
67730 M-1 cm-1 |
| assuming all Cys residues are reduced |
67730 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
69105 M-1 cm-1 |
|
|
| Codon Bias |
0.043 |
| Codon Adaptation Index |
0.133 |
| Frequency of Optimal Codons |
0.434 |
| Hydropathicity of Protein |
-0.184 |
| Aromaticity Score |
0.067 |
|
