RPS28B/YLR264W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 2 3.0%
Arg[R] 10 14.9%
Asn[N] 2 3.0%
Asp[D] 3 4.5%
Cys[C] 0 0.0%
Gln[Q] 1 1.5%
Glu[E] 6 9.0%
Gly[G] 5 7.5%
His[H] 0 0.0%
Ile[I] 3 4.5%
Leu[L] 6 9.0%
Lys[K] 4 6.0%
Met[M] 2 3.0%
Phe[F] 1 1.5%
Pro[P] 2 3.0%
Ser[S] 4 6.0%
Thr[T] 6 9.0%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 10 14.9%
Total Length 67

Instability index
Instability index (II) 34.50
This classifies the protein as stable

Atomic composition
Carbon 324
Hydrogen 564
Nitrogen 104
Oxygen 99
Sulfur 2
Total number of atoms 1093
Formula = C324H564N104O99S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours


Aliphatic index
Aliphatic index 98.66

Coding Region Translation Calculations
Codon Bias 0.602
Codon Adaptation Index 0.561
Frequency of Optimal Codons 0.754
Hydropathicity of Protein -0.399
Aromaticity Score 0.015