HSP60/YLR259C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 56 9.8%
Arg[R] 28 4.9%
Asn[N] 17 3.0%
Asp[D] 32 5.6%
Cys[C] 5 0.9%
Gln[Q] 13 2.3%
Glu[E] 51 8.9%
Gly[G] 56 9.8%
His[H] 2 0.3%
Ile[I] 42 7.3%
Leu[L] 49 8.6%
Lys[K] 44 7.7%
Met[M] 11 1.9%
Phe[F] 14 2.4%
Pro[P] 23 4.0%
Ser[S] 38 6.6%
Thr[T] 35 6.1%
Trp[W] 0 0.0%
Tyr[Y] 7 1.2%
Val[V] 49 8.6%
Total Length 572

Instability index
Instability index (II) 39.52
This classifies the protein as stable

Atomic composition
Carbon 2659
Hydrogen 4402
Nitrogen 734
Oxygen 849
Sulfur 16
Total number of atoms 8660
Formula = C2659H4402N734O849S16

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 10680 M-1 cm-1
assuming NO Cys residues appear as half cystines 10430 M-1 cm-1
assuming all Cys residues are reduced 10430 M-1 cm-1
assuming all pairs of Cys residues form cystines 10680 M-1 cm-1

Aliphatic index
Aliphatic index 96.68

Coding Region Translation Calculations
Codon Bias 0.478
Codon Adaptation Index 0.382
Frequency of Optimal Codons 0.684
Hydropathicity of Protein -0.119
Aromaticity Score 0.037