Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
3.3% |
| Arg[R] |
44 |
5.0% |
| Asn[N] |
58 |
6.6% |
| Asp[D] |
54 |
6.2% |
| Cys[C] |
11 |
1.3% |
| Gln[Q] |
29 |
3.3% |
| Glu[E] |
54 |
6.2% |
| Gly[G] |
31 |
3.5% |
| His[H] |
16 |
1.8% |
| Ile[I] |
61 |
7.0% |
| Leu[L] |
121 |
13.8% |
| Lys[K] |
66 |
7.5% |
| Met[M] |
14 |
1.6% |
| Phe[F] |
39 |
4.5% |
| Pro[P] |
49 |
5.6% |
| Ser[S] |
66 |
7.5% |
| Thr[T] |
44 |
5.0% |
| Trp[W] |
11 |
1.3% |
| Tyr[Y] |
31 |
3.5% |
| Val[V] |
47 |
5.4% |
| Total Length |
875 |
|
| Instability index (II) |
40.58 |
| This classifies the protein as unstable |
|
| Carbon |
4568 |
| Hydrogen |
7227 |
| Nitrogen |
1203 |
| Oxygen |
1320 |
| Sulfur |
25 |
| Total number of atoms |
14343 |
| Formula = C4568H7227N1203O1320S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
107315 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
106690 M-1 cm-1 |
| assuming all Cys residues are reduced |
106690 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
107315 M-1 cm-1 |
|
|
| Codon Bias |
0.030 |
| Codon Adaptation Index |
0.129 |
| Frequency of Optimal Codons |
0.420 |
| Hydropathicity of Protein |
-0.305 |
| Aromaticity Score |
0.093 |
|
