CPR6/YLR216C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 26 7.0%
Arg[R] 9 2.4%
Asn[N] 24 6.5%
Asp[D] 29 7.8%
Cys[C] 7 1.9%
Gln[Q] 17 4.6%
Glu[E] 28 7.5%
Gly[G] 19 5.1%
His[H] 6 1.6%
Ile[I] 21 5.7%
Leu[L] 29 7.8%
Lys[K] 43 11.6%
Met[M] 7 1.9%
Phe[F] 20 5.4%
Pro[P] 16 4.3%
Ser[S] 13 3.5%
Thr[T] 19 5.1%
Trp[W] 0 0.0%
Tyr[Y] 14 3.8%
Val[V] 24 6.5%
Total Length 371

Instability index
Instability index (II) 26.89
This classifies the protein as stable

Atomic composition
Carbon 1878
Hydrogen 2956
Nitrogen 494
Oxygen 573
Sulfur 14
Total number of atoms 5915
Formula = C1878H2956N494O573S14

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 21235 M-1 cm-1
assuming NO Cys residues appear as half cystines 20860 M-1 cm-1
assuming all Cys residues are reduced 20860 M-1 cm-1
assuming all pairs of Cys residues form cystines 21235 M-1 cm-1

Aliphatic index
Aliphatic index 78.33

Coding Region Translation Calculations
Codon Bias 0.195
Codon Adaptation Index 0.253
Frequency of Optimal Codons 0.538
Hydropathicity of Protein -0.556
Aromaticity Score 0.092