Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
41 |
6.0% |
| Arg[R] |
31 |
4.5% |
| Asn[N] |
37 |
5.4% |
| Asp[D] |
23 |
3.4% |
| Cys[C] |
17 |
2.5% |
| Gln[Q] |
15 |
2.2% |
| Glu[E] |
30 |
4.4% |
| Gly[G] |
30 |
4.4% |
| His[H] |
16 |
2.3% |
| Ile[I] |
48 |
7.0% |
| Leu[L] |
64 |
9.3% |
| Lys[K] |
47 |
6.9% |
| Met[M] |
19 |
2.8% |
| Phe[F] |
46 |
6.7% |
| Pro[P] |
22 |
3.2% |
| Ser[S] |
62 |
9.0% |
| Thr[T] |
37 |
5.4% |
| Trp[W] |
16 |
2.3% |
| Tyr[Y] |
33 |
4.8% |
| Val[V] |
52 |
7.6% |
| Total Length |
686 |
|
| Instability index (II) |
51.63 |
| This classifies the protein as unstable |
|
| Carbon |
3621 |
| Hydrogen |
5562 |
| Nitrogen |
926 |
| Oxygen |
977 |
| Sulfur |
36 |
| Total number of atoms |
11122 |
| Formula = C3621H5562N926O977S36 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
138170 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
137170 M-1 cm-1 |
| assuming all Cys residues are reduced |
137170 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
138170 M-1 cm-1 |
|
|
| Codon Bias |
0.054 |
| Codon Adaptation Index |
0.145 |
| Frequency of Optimal Codons |
0.444 |
| Hydropathicity of Protein |
0.054 |
| Aromaticity Score |
0.138 |
|
