Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
17 |
3.5% |
| Arg[R] |
30 |
6.1% |
| Asn[N] |
29 |
5.9% |
| Asp[D] |
27 |
5.5% |
| Cys[C] |
8 |
1.6% |
| Gln[Q] |
20 |
4.1% |
| Glu[E] |
28 |
5.7% |
| Gly[G] |
21 |
4.3% |
| His[H] |
12 |
2.5% |
| Ile[I] |
24 |
4.9% |
| Leu[L] |
38 |
7.8% |
| Lys[K] |
44 |
9.0% |
| Met[M] |
11 |
2.2% |
| Phe[F] |
20 |
4.1% |
| Pro[P] |
30 |
6.1% |
| Ser[S] |
64 |
13.1% |
| Thr[T] |
23 |
4.7% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
17 |
3.5% |
| Val[V] |
25 |
5.1% |
| Total Length |
489 |
|
| Instability index (II) |
56.61 |
| This classifies the protein as unstable |
|
| Carbon |
2427 |
| Hydrogen |
3867 |
| Nitrogen |
697 |
| Oxygen |
753 |
| Sulfur |
19 |
| Total number of atoms |
7763 |
| Formula = C2427H3867N697O753S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
31330 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
30830 M-1 cm-1 |
| assuming all Cys residues are reduced |
30830 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
31330 M-1 cm-1 |
|
|
| Codon Bias |
0.087 |
| Codon Adaptation Index |
0.148 |
| Frequency of Optimal Codons |
0.455 |
| Hydropathicity of Protein |
-0.758 |
| Aromaticity Score |
0.078 |
|
