Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
37 |
4.6% |
| Arg[R] |
25 |
3.1% |
| Asn[N] |
71 |
8.8% |
| Asp[D] |
68 |
8.5% |
| Cys[C] |
6 |
0.7% |
| Gln[Q] |
37 |
4.6% |
| Glu[E] |
72 |
9.0% |
| Gly[G] |
40 |
5.0% |
| His[H] |
18 |
2.2% |
| Ile[I] |
47 |
5.9% |
| Leu[L] |
96 |
12.0% |
| Lys[K] |
62 |
7.7% |
| Met[M] |
17 |
2.1% |
| Phe[F] |
22 |
2.7% |
| Pro[P] |
33 |
4.1% |
| Ser[S] |
66 |
8.2% |
| Thr[T] |
38 |
4.7% |
| Trp[W] |
5 |
0.6% |
| Tyr[Y] |
17 |
2.1% |
| Val[V] |
26 |
3.2% |
| Total Length |
803 |
|
| Instability index (II) |
45.65 |
| This classifies the protein as unstable |
|
| Carbon |
3934 |
| Hydrogen |
6261 |
| Nitrogen |
1089 |
| Oxygen |
1313 |
| Sulfur |
23 |
| Total number of atoms |
12620 |
| Formula = C3934H6261N1089O1313S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
53205 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
52830 M-1 cm-1 |
| assuming all Cys residues are reduced |
52830 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
53205 M-1 cm-1 |
|
|
| Codon Bias |
0.012 |
| Codon Adaptation Index |
0.135 |
| Frequency of Optimal Codons |
0.416 |
| Hydropathicity of Protein |
-0.739 |
| Aromaticity Score |
0.055 |
|
