Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
56 |
8.2% |
| Arg[R] |
31 |
4.5% |
| Asn[N] |
27 |
4.0% |
| Asp[D] |
40 |
5.9% |
| Cys[C] |
6 |
0.9% |
| Gln[Q] |
18 |
2.6% |
| Glu[E] |
40 |
5.9% |
| Gly[G] |
58 |
8.5% |
| His[H] |
22 |
3.2% |
| Ile[I] |
42 |
6.1% |
| Leu[L] |
49 |
7.2% |
| Lys[K] |
40 |
5.9% |
| Met[M] |
11 |
1.6% |
| Phe[F] |
20 |
2.9% |
| Pro[P] |
36 |
5.3% |
| Ser[S] |
41 |
6.0% |
| Thr[T] |
42 |
6.1% |
| Trp[W] |
13 |
1.9% |
| Tyr[Y] |
31 |
4.5% |
| Val[V] |
60 |
8.8% |
| Total Length |
683 |
|
| Instability index (II) |
32.03 |
| This classifies the protein as stable |
|
| Carbon |
3392 |
| Hydrogen |
5258 |
| Nitrogen |
918 |
| Oxygen |
1003 |
| Sulfur |
17 |
| Total number of atoms |
10588 |
| Formula = C3392H5258N918O1003S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
118065 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
117690 M-1 cm-1 |
| assuming all Cys residues are reduced |
117690 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
118065 M-1 cm-1 |
|
|
| Codon Bias |
0.421 |
| Codon Adaptation Index |
0.371 |
| Frequency of Optimal Codons |
0.654 |
| Hydropathicity of Protein |
-0.261 |
| Aromaticity Score |
0.094 |
|
