Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
23 |
3.0% |
| Arg[R] |
38 |
4.9% |
| Asn[N] |
73 |
9.5% |
| Asp[D] |
54 |
7.0% |
| Cys[C] |
8 |
1.0% |
| Gln[Q] |
33 |
4.3% |
| Glu[E] |
38 |
4.9% |
| Gly[G] |
36 |
4.7% |
| His[H] |
28 |
3.6% |
| Ile[I] |
41 |
5.3% |
| Leu[L] |
71 |
9.2% |
| Lys[K] |
59 |
7.7% |
| Met[M] |
15 |
1.9% |
| Phe[F] |
17 |
2.2% |
| Pro[P] |
62 |
8.1% |
| Ser[S] |
73 |
9.5% |
| Thr[T] |
49 |
6.4% |
| Trp[W] |
2 |
0.3% |
| Tyr[Y] |
20 |
2.6% |
| Val[V] |
30 |
3.9% |
| Total Length |
770 |
|
| Instability index (II) |
53.52 |
| This classifies the protein as unstable |
|
| Carbon |
3755 |
| Hydrogen |
5995 |
| Nitrogen |
1107 |
| Oxygen |
1203 |
| Sulfur |
23 |
| Total number of atoms |
12083 |
| Formula = C3755H5995N1107O1203S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
41300 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
40800 M-1 cm-1 |
| assuming all Cys residues are reduced |
40800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
41300 M-1 cm-1 |
|
|
| Codon Bias |
0.001 |
| Codon Adaptation Index |
0.119 |
| Frequency of Optimal Codons |
0.405 |
| Hydropathicity of Protein |
-0.909 |
| Aromaticity Score |
0.051 |
|
