Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
53 |
5.6% |
| Arg[R] |
30 |
3.2% |
| Asn[N] |
35 |
3.7% |
| Asp[D] |
68 |
7.2% |
| Cys[C] |
13 |
1.4% |
| Gln[Q] |
33 |
3.5% |
| Glu[E] |
68 |
7.2% |
| Gly[G] |
58 |
6.2% |
| His[H] |
24 |
2.5% |
| Ile[I] |
65 |
6.9% |
| Leu[L] |
105 |
11.1% |
| Lys[K] |
85 |
9.0% |
| Met[M] |
15 |
1.6% |
| Phe[F] |
41 |
4.3% |
| Pro[P] |
21 |
2.2% |
| Ser[S] |
79 |
8.4% |
| Thr[T] |
59 |
6.3% |
| Trp[W] |
19 |
2.0% |
| Tyr[Y] |
22 |
2.3% |
| Val[V] |
50 |
5.3% |
| Total Length |
943 |
|
| Instability index (II) |
41.24 |
| This classifies the protein as unstable |
|
| Carbon |
4764 |
| Hydrogen |
7511 |
| Nitrogen |
1253 |
| Oxygen |
1444 |
| Sulfur |
28 |
| Total number of atoms |
15000 |
| Formula = C4764H7511N1253O1444S28 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
138030 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
137280 M-1 cm-1 |
| assuming all Cys residues are reduced |
137280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
138030 M-1 cm-1 |
|
|
| Codon Bias |
0.082 |
| Codon Adaptation Index |
0.166 |
| Frequency of Optimal Codons |
0.460 |
| Hydropathicity of Protein |
-0.309 |
| Aromaticity Score |
0.087 |
|
