Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
3.2% |
| Arg[R] |
35 |
4.1% |
| Asn[N] |
69 |
8.1% |
| Asp[D] |
56 |
6.6% |
| Cys[C] |
12 |
1.4% |
| Gln[Q] |
28 |
3.3% |
| Glu[E] |
54 |
6.3% |
| Gly[G] |
22 |
2.6% |
| His[H] |
20 |
2.3% |
| Ile[I] |
63 |
7.4% |
| Leu[L] |
113 |
13.2% |
| Lys[K] |
67 |
7.9% |
| Met[M] |
18 |
2.1% |
| Phe[F] |
47 |
5.5% |
| Pro[P] |
31 |
3.6% |
| Ser[S] |
56 |
6.6% |
| Thr[T] |
50 |
5.9% |
| Trp[W] |
13 |
1.5% |
| Tyr[Y] |
34 |
4.0% |
| Val[V] |
38 |
4.5% |
| Total Length |
853 |
|
| Instability index (II) |
37.04 |
| This classifies the protein as stable |
|
| Carbon |
4534 |
| Hydrogen |
7069 |
| Nitrogen |
1175 |
| Oxygen |
1311 |
| Sulfur |
30 |
| Total number of atoms |
14119 |
| Formula = C4534H7069N1175O1311S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
122910 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
122160 M-1 cm-1 |
| assuming all Cys residues are reduced |
122160 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
122910 M-1 cm-1 |
|
|
| Codon Bias |
-0.052 |
| Codon Adaptation Index |
0.125 |
| Frequency of Optimal Codons |
0.387 |
| Hydropathicity of Protein |
-0.334 |
| Aromaticity Score |
0.110 |
|
