MDN1/YLR106C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 263 5.4%
Arg[R] 195 4.0%
Asn[N] 288 5.9%
Asp[D] 362 7.4%
Cys[C] 39 0.8%
Gln[Q] 179 3.6%
Glu[E] 435 8.9%
Gly[G] 210 4.3%
His[H] 79 1.6%
Ile[I] 343 7.0%
Leu[L] 565 11.5%
Lys[K] 369 7.5%
Met[M] 96 2.0%
Phe[F] 226 4.6%
Pro[P] 157 3.2%
Ser[S] 400 8.1%
Thr[T] 263 5.4%
Trp[W] 53 1.1%
Tyr[Y] 137 2.8%
Val[V] 251 5.1%
Total Length 4910

Instability index
Instability index (II) 41.92
This classifies the protein as unstable

Atomic composition
Carbon 24924
Hydrogen 39326
Nitrogen 6542
Oxygen 7772
Sulfur 135
Total number of atoms 78699
Formula = C24924H39326N6542O7772S135

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 498005 M-1 cm-1
assuming NO Cys residues appear as half cystines 495630 M-1 cm-1
assuming all Cys residues are reduced 495630 M-1 cm-1
assuming all pairs of Cys residues form cystines 498005 M-1 cm-1

Aliphatic index
Aliphatic index 92.30

Coding Region Translation Calculations
Codon Bias 0.085
Codon Adaptation Index 0.161
Frequency of Optimal Codons 0.462
Hydropathicity of Protein -0.393
Aromaticity Score 0.085