Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
4.5% |
| Arg[R] |
34 |
5.2% |
| Asn[N] |
41 |
6.3% |
| Asp[D] |
59 |
9.1% |
| Cys[C] |
7 |
1.1% |
| Gln[Q] |
28 |
4.3% |
| Glu[E] |
48 |
7.4% |
| Gly[G] |
35 |
5.4% |
| His[H] |
9 |
1.4% |
| Ile[I] |
45 |
6.9% |
| Leu[L] |
86 |
13.2% |
| Lys[K] |
33 |
5.1% |
| Met[M] |
7 |
1.1% |
| Phe[F] |
21 |
3.2% |
| Pro[P] |
16 |
2.5% |
| Ser[S] |
54 |
8.3% |
| Thr[T] |
33 |
5.1% |
| Trp[W] |
8 |
1.2% |
| Tyr[Y] |
29 |
4.5% |
| Val[V] |
28 |
4.3% |
| Total Length |
650 |
|
| Instability index (II) |
45.86 |
| This classifies the protein as unstable |
|
| Carbon |
3287 |
| Hydrogen |
5154 |
| Nitrogen |
880 |
| Oxygen |
1050 |
| Sulfur |
14 |
| Total number of atoms |
10385 |
| Formula = C3287H5154N880O1050S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
87585 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
87210 M-1 cm-1 |
| assuming all Cys residues are reduced |
87210 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
87585 M-1 cm-1 |
|
|
| Codon Bias |
0.045 |
| Codon Adaptation Index |
0.129 |
| Frequency of Optimal Codons |
0.430 |
| Hydropathicity of Protein |
-0.444 |
| Aromaticity Score |
0.089 |
|
