Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
4.7% |
| Arg[R] |
41 |
5.0% |
| Asn[N] |
49 |
6.0% |
| Asp[D] |
51 |
6.3% |
| Cys[C] |
12 |
1.5% |
| Gln[Q] |
52 |
6.4% |
| Glu[E] |
68 |
8.4% |
| Gly[G] |
29 |
3.6% |
| His[H] |
20 |
2.5% |
| Ile[I] |
58 |
7.1% |
| Leu[L] |
68 |
8.4% |
| Lys[K] |
56 |
6.9% |
| Met[M] |
12 |
1.5% |
| Phe[F] |
29 |
3.6% |
| Pro[P] |
47 |
5.8% |
| Ser[S] |
66 |
8.1% |
| Thr[T] |
46 |
5.7% |
| Trp[W] |
13 |
1.6% |
| Tyr[Y] |
23 |
2.8% |
| Val[V] |
34 |
4.2% |
| Total Length |
812 |
|
| Instability index (II) |
55.69 |
| This classifies the protein as unstable |
|
| Carbon |
4124 |
| Hydrogen |
6463 |
| Nitrogen |
1145 |
| Oxygen |
1287 |
| Sulfur |
24 |
| Total number of atoms |
13043 |
| Formula = C4124H6463N1145O1287S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
106520 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
105770 M-1 cm-1 |
| assuming all Cys residues are reduced |
105770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
106520 M-1 cm-1 |
|
|
| Codon Bias |
0.041 |
| Codon Adaptation Index |
0.120 |
| Frequency of Optimal Codons |
0.440 |
| Hydropathicity of Protein |
-0.721 |
| Aromaticity Score |
0.080 |
|
