Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
41 |
6.7% |
| Arg[R] |
23 |
3.7% |
| Asn[N] |
32 |
5.2% |
| Asp[D] |
19 |
3.1% |
| Cys[C] |
6 |
1.0% |
| Gln[Q] |
16 |
2.6% |
| Glu[E] |
36 |
5.9% |
| Gly[G] |
31 |
5.0% |
| His[H] |
13 |
2.1% |
| Ile[I] |
48 |
7.8% |
| Leu[L] |
78 |
12.7% |
| Lys[K] |
23 |
3.7% |
| Met[M] |
19 |
3.1% |
| Phe[F] |
40 |
6.5% |
| Pro[P] |
24 |
3.9% |
| Ser[S] |
66 |
10.7% |
| Thr[T] |
27 |
4.4% |
| Trp[W] |
15 |
2.4% |
| Tyr[Y] |
18 |
2.9% |
| Val[V] |
39 |
6.4% |
| Total Length |
614 |
|
| Instability index (II) |
44.17 |
| This classifies the protein as unstable |
|
| Carbon |
3180 |
| Hydrogen |
4907 |
| Nitrogen |
795 |
| Oxygen |
884 |
| Sulfur |
25 |
| Total number of atoms |
9791 |
| Formula = C3180H4907N795O884S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
109695 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
109320 M-1 cm-1 |
| assuming all Cys residues are reduced |
109320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
109695 M-1 cm-1 |
|
|
| Codon Bias |
0.048 |
| Codon Adaptation Index |
0.121 |
| Frequency of Optimal Codons |
0.424 |
| Hydropathicity of Protein |
0.258 |
| Aromaticity Score |
0.119 |
|
