Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
64 |
4.5% |
| Arg[R] |
74 |
5.2% |
| Asn[N] |
79 |
5.6% |
| Asp[D] |
84 |
5.9% |
| Cys[C] |
9 |
0.6% |
| Gln[Q] |
71 |
5.0% |
| Glu[E] |
159 |
11.2% |
| Gly[G] |
53 |
3.7% |
| His[H] |
22 |
1.6% |
| Ile[I] |
72 |
5.1% |
| Leu[L] |
163 |
11.5% |
| Lys[K] |
151 |
10.6% |
| Met[M] |
33 |
2.3% |
| Phe[F] |
39 |
2.8% |
| Pro[P] |
37 |
2.6% |
| Ser[S] |
131 |
9.2% |
| Thr[T] |
65 |
4.6% |
| Trp[W] |
2 |
0.1% |
| Tyr[Y] |
30 |
2.1% |
| Val[V] |
80 |
5.6% |
| Total Length |
1418 |
|
| Instability index (II) |
46.78 |
| This classifies the protein as unstable |
|
| Carbon |
7065 |
| Hydrogen |
11567 |
| Nitrogen |
1987 |
| Oxygen |
2281 |
| Sulfur |
42 |
| Total number of atoms |
22942 |
| Formula = C7065H11567N1987O2281S42 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
56200 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
55700 M-1 cm-1 |
| assuming all Cys residues are reduced |
55700 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
56200 M-1 cm-1 |
|
|
| Codon Bias |
0.084 |
| Codon Adaptation Index |
0.147 |
| Frequency of Optimal Codons |
0.458 |
| Hydropathicity of Protein |
-0.741 |
| Aromaticity Score |
0.050 |
|
