Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
15 |
3.8% |
| Arg[R] |
22 |
5.6% |
| Asn[N] |
31 |
7.9% |
| Asp[D] |
25 |
6.4% |
| Cys[C] |
3 |
0.8% |
| Gln[Q] |
13 |
3.3% |
| Glu[E] |
35 |
8.9% |
| Gly[G] |
14 |
3.6% |
| His[H] |
8 |
2.0% |
| Ile[I] |
26 |
6.6% |
| Leu[L] |
33 |
8.4% |
| Lys[K] |
39 |
9.9% |
| Met[M] |
8 |
2.0% |
| Phe[F] |
14 |
3.6% |
| Pro[P] |
10 |
2.6% |
| Ser[S] |
31 |
7.9% |
| Thr[T] |
28 |
7.1% |
| Trp[W] |
4 |
1.0% |
| Tyr[Y] |
8 |
2.0% |
| Val[V] |
25 |
6.4% |
| Total Length |
392 |
|
| Instability index (II) |
41.13 |
| This classifies the protein as unstable |
|
| Carbon |
1976 |
| Hydrogen |
3187 |
| Nitrogen |
561 |
| Oxygen |
624 |
| Sulfur |
11 |
| Total number of atoms |
6359 |
| Formula = C1976H3187N561O624S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
34045 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
33920 M-1 cm-1 |
| assuming all Cys residues are reduced |
33920 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
34045 M-1 cm-1 |
|
|
| Codon Bias |
0.022 |
| Codon Adaptation Index |
0.130 |
| Frequency of Optimal Codons |
0.437 |
| Hydropathicity of Protein |
-0.726 |
| Aromaticity Score |
0.066 |
|
