GAL2/YLR081W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 38 6.6%
Arg[R] 15 2.6%
Asn[N] 20 3.5%
Asp[D] 16 2.8%
Cys[C] 15 2.6%
Gln[Q] 19 3.3%
Glu[E] 29 5.1%
Gly[G] 52 9.1%
His[H] 7 1.2%
Ile[I] 40 7.0%
Leu[L] 49 8.5%
Lys[K] 30 5.2%
Met[M] 20 3.5%
Phe[F] 38 6.6%
Pro[P] 21 3.7%
Ser[S] 51 8.9%
Thr[T] 27 4.7%
Trp[W] 14 2.4%
Tyr[Y] 25 4.4%
Val[V] 48 8.4%
Total Length 574

Instability index
Instability index (II) 42.04
This classifies the protein as unstable

Atomic composition
Carbon 2920
Hydrogen 4456
Nitrogen 716
Oxygen 807
Sulfur 35
Total number of atoms 8934
Formula = C2920H4456N716O807S35

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 115125 M-1 cm-1
assuming NO Cys residues appear as half cystines 114250 M-1 cm-1
assuming all Cys residues are reduced 114250 M-1 cm-1
assuming all pairs of Cys residues form cystines 115125 M-1 cm-1

Aliphatic index
Aliphatic index 91.34

Coding Region Translation Calculations
Codon Bias 0.277
Codon Adaptation Index 0.245
Frequency of Optimal Codons 0.570
Hydropathicity of Protein 0.275
Aromaticity Score 0.134