Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
44 |
4.1% |
| Arg[R] |
41 |
3.8% |
| Asn[N] |
96 |
8.9% |
| Asp[D] |
42 |
3.9% |
| Cys[C] |
9 |
0.8% |
| Gln[Q] |
53 |
4.9% |
| Glu[E] |
62 |
5.7% |
| Gly[G] |
37 |
3.4% |
| His[H] |
27 |
2.5% |
| Ile[I] |
96 |
8.9% |
| Leu[L] |
126 |
11.6% |
| Lys[K] |
75 |
6.9% |
| Met[M] |
33 |
3.0% |
| Phe[F] |
41 |
3.8% |
| Pro[P] |
43 |
4.0% |
| Ser[S] |
92 |
8.5% |
| Thr[T] |
69 |
6.4% |
| Trp[W] |
9 |
0.8% |
| Tyr[Y] |
30 |
2.8% |
| Val[V] |
57 |
5.3% |
| Total Length |
1082 |
|
| Instability index (II) |
39.18 |
| This classifies the protein as stable |
|
| Carbon |
5505 |
| Hydrogen |
8828 |
| Nitrogen |
1492 |
| Oxygen |
1631 |
| Sulfur |
42 |
| Total number of atoms |
17498 |
| Formula = C5505H8828N1492O1631S42 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
94700 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
94200 M-1 cm-1 |
| assuming all Cys residues are reduced |
94200 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
94700 M-1 cm-1 |
|
|
| Codon Bias |
0.043 |
| Codon Adaptation Index |
0.135 |
| Frequency of Optimal Codons |
0.442 |
| Hydropathicity of Protein |
-0.251 |
| Aromaticity Score |
0.074 |
|
