ERG3/YLR056W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 18 4.9%
Arg[R] 14 3.8%
Asn[N] 20 5.5%
Asp[D] 20 5.5%
Cys[C] 4 1.1%
Gln[Q] 8 2.2%
Glu[E] 14 3.8%
Gly[G] 14 3.8%
His[H] 18 4.9%
Ile[I] 19 5.2%
Leu[L] 44 12.1%
Lys[K] 20 5.5%
Met[M] 9 2.5%
Phe[F] 26 7.1%
Pro[P] 17 4.7%
Ser[S] 25 6.8%
Thr[T] 18 4.9%
Trp[W] 10 2.7%
Tyr[Y] 20 5.5%
Val[V] 27 7.4%
Total Length 365

Instability index
Instability index (II) 38.95
This classifies the protein as stable

Atomic composition
Carbon 1990
Hydrogen 2971
Nitrogen 501
Oxygen 525
Sulfur 13
Total number of atoms 6000
Formula = C1990H2971N501O525S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 85050 M-1 cm-1
assuming NO Cys residues appear as half cystines 84800 M-1 cm-1
assuming all Cys residues are reduced 84800 M-1 cm-1
assuming all pairs of Cys residues form cystines 85050 M-1 cm-1

Aliphatic index
Aliphatic index 93.70

Coding Region Translation Calculations
Codon Bias 0.304
Codon Adaptation Index 0.249
Frequency of Optimal Codons 0.587
Hydropathicity of Protein -0.048
Aromaticity Score 0.153