Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
5.8% |
| Arg[R] |
21 |
3.5% |
| Asn[N] |
37 |
6.1% |
| Asp[D] |
68 |
11.3% |
| Cys[C] |
6 |
1.0% |
| Gln[Q] |
22 |
3.7% |
| Glu[E] |
55 |
9.1% |
| Gly[G] |
52 |
8.6% |
| His[H] |
15 |
2.5% |
| Ile[I] |
32 |
5.3% |
| Leu[L] |
49 |
8.1% |
| Lys[K] |
29 |
4.8% |
| Met[M] |
13 |
2.2% |
| Phe[F] |
10 |
1.7% |
| Pro[P] |
19 |
3.2% |
| Ser[S] |
58 |
9.6% |
| Thr[T] |
23 |
3.8% |
| Trp[W] |
7 |
1.2% |
| Tyr[Y] |
18 |
3.0% |
| Val[V] |
33 |
5.5% |
| Total Length |
602 |
|
| Instability index (II) |
46.24 |
| This classifies the protein as unstable |
|
| Carbon |
2828 |
| Hydrogen |
4402 |
| Nitrogen |
790 |
| Oxygen |
1007 |
| Sulfur |
19 |
| Total number of atoms |
9046 |
| Formula = C2828H4402N790O1007S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
65695 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
65320 M-1 cm-1 |
| assuming all Cys residues are reduced |
65320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
65695 M-1 cm-1 |
|
|
| Codon Bias |
0.061 |
| Codon Adaptation Index |
0.124 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-0.725 |
| Aromaticity Score |
0.058 |
|
