Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
54 |
9.6% |
| Arg[R] |
15 |
2.7% |
| Asn[N] |
29 |
5.2% |
| Asp[D] |
29 |
5.2% |
| Cys[C] |
4 |
0.7% |
| Gln[Q] |
22 |
3.9% |
| Glu[E] |
30 |
5.3% |
| Gly[G] |
42 |
7.5% |
| His[H] |
12 |
2.1% |
| Ile[I] |
37 |
6.6% |
| Leu[L] |
54 |
9.6% |
| Lys[K] |
35 |
6.2% |
| Met[M] |
13 |
2.3% |
| Phe[F] |
23 |
4.1% |
| Pro[P] |
26 |
4.6% |
| Ser[S] |
30 |
5.3% |
| Thr[T] |
44 |
7.8% |
| Trp[W] |
7 |
1.2% |
| Tyr[Y] |
17 |
3.0% |
| Val[V] |
40 |
7.1% |
| Total Length |
563 |
|
| Instability index (II) |
30.84 |
| This classifies the protein as stable |
|
| Carbon |
2766 |
| Hydrogen |
4355 |
| Nitrogen |
725 |
| Oxygen |
824 |
| Sulfur |
17 |
| Total number of atoms |
8687 |
| Formula = C2766H4355N725O824S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
64080 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
63830 M-1 cm-1 |
| assuming all Cys residues are reduced |
63830 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
64080 M-1 cm-1 |
|
|
| Codon Bias |
0.914 |
| Codon Adaptation Index |
0.904 |
| Frequency of Optimal Codons |
0.948 |
| Hydropathicity of Protein |
-0.059 |
| Aromaticity Score |
0.083 |
|
