Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
17 |
7.1% |
| Arg[R] |
8 |
3.3% |
| Asn[N] |
19 |
7.9% |
| Asp[D] |
14 |
5.8% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
12 |
5.0% |
| Glu[E] |
35 |
14.6% |
| Gly[G] |
9 |
3.8% |
| His[H] |
3 |
1.2% |
| Ile[I] |
15 |
6.2% |
| Leu[L] |
22 |
9.2% |
| Lys[K] |
22 |
9.2% |
| Met[M] |
10 |
4.2% |
| Phe[F] |
3 |
1.2% |
| Pro[P] |
5 |
2.1% |
| Ser[S] |
21 |
8.8% |
| Thr[T] |
12 |
5.0% |
| Trp[W] |
2 |
0.8% |
| Tyr[Y] |
0 |
0.0% |
| Val[V] |
11 |
4.6% |
| Total Length |
240 |
|
| Instability index (II) |
50.28 |
| This classifies the protein as unstable |
|
| Carbon |
1146 |
| Hydrogen |
1887 |
| Nitrogen |
325 |
| Oxygen |
403 |
| Sulfur |
10 |
| Total number of atoms |
3771 |
| Formula = C1146H1887N325O403S10 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
11000 M-1 cm-1 |
|
|
| Codon Bias |
-0.002 |
| Codon Adaptation Index |
0.114 |
| Frequency of Optimal Codons |
0.417 |
| Hydropathicity of Protein |
-0.811 |
| Aromaticity Score |
0.021 |
|
