FRE6/YLL051C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 37 5.2%
Arg[R] 28 3.9%
Asn[N] 45 6.3%
Asp[D] 25 3.5%
Cys[C] 13 1.8%
Gln[Q] 16 2.2%
Glu[E] 26 3.7%
Gly[G] 31 4.4%
His[H] 29 4.1%
Ile[I] 67 9.4%
Leu[L] 83 11.7%
Lys[K] 38 5.3%
Met[M] 8 1.1%
Phe[F] 55 7.7%
Pro[P] 28 3.9%
Ser[S] 62 8.7%
Thr[T] 35 4.9%
Trp[W] 15 2.1%
Tyr[Y] 31 4.4%
Val[V] 40 5.6%
Total Length 712

Instability index
Instability index (II) 33.75
This classifies the protein as stable

Atomic composition
Carbon 3817
Hydrogen 5802
Nitrogen 968
Oxygen 1004
Sulfur 21
Total number of atoms 11612
Formula = C3817H5802N968O1004S21

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 129440 M-1 cm-1
assuming NO Cys residues appear as half cystines 128690 M-1 cm-1
assuming all Cys residues are reduced 128690 M-1 cm-1
assuming all pairs of Cys residues form cystines 129440 M-1 cm-1

Aliphatic index
Aliphatic index 103.65

Coding Region Translation Calculations
Codon Bias -0.008
Codon Adaptation Index 0.108
Frequency of Optimal Codons 0.408
Hydropathicity of Protein 0.153
Aromaticity Score 0.142