Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
7.7% |
| Arg[R] |
36 |
6.5% |
| Asn[N] |
16 |
2.9% |
| Asp[D] |
33 |
5.9% |
| Cys[C] |
3 |
0.5% |
| Gln[Q] |
23 |
4.1% |
| Glu[E] |
57 |
10.2% |
| Gly[G] |
32 |
5.7% |
| His[H] |
12 |
2.2% |
| Ile[I] |
28 |
5.0% |
| Leu[L] |
56 |
10.1% |
| Lys[K] |
48 |
8.6% |
| Met[M] |
11 |
2.0% |
| Phe[F] |
28 |
5.0% |
| Pro[P] |
26 |
4.7% |
| Ser[S] |
28 |
5.0% |
| Thr[T] |
25 |
4.5% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
18 |
3.2% |
| Val[V] |
33 |
5.9% |
| Total Length |
557 |
|
| Instability index (II) |
46.07 |
| This classifies the protein as unstable |
|
| Carbon |
2837 |
| Hydrogen |
4505 |
| Nitrogen |
777 |
| Oxygen |
848 |
| Sulfur |
14 |
| Total number of atoms |
8981 |
| Formula = C2837H4505N777O848S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
32445 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
32320 M-1 cm-1 |
| assuming all Cys residues are reduced |
32320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
32445 M-1 cm-1 |
|
|
| Codon Bias |
0.464 |
| Codon Adaptation Index |
0.350 |
| Frequency of Optimal Codons |
0.677 |
| Hydropathicity of Protein |
-0.532 |
| Aromaticity Score |
0.084 |
|
