Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
52 |
5.9% |
| Arg[R] |
22 |
2.5% |
| Asn[N] |
96 |
10.9% |
| Asp[D] |
37 |
4.2% |
| Cys[C] |
3 |
0.3% |
| Gln[Q] |
68 |
7.7% |
| Glu[E] |
48 |
5.5% |
| Gly[G] |
44 |
5.0% |
| His[H] |
26 |
3.0% |
| Ile[I] |
51 |
5.8% |
| Leu[L] |
61 |
6.9% |
| Lys[K] |
59 |
6.7% |
| Met[M] |
19 |
2.2% |
| Phe[F] |
37 |
4.2% |
| Pro[P] |
49 |
5.6% |
| Ser[S] |
103 |
11.7% |
| Thr[T] |
31 |
3.5% |
| Trp[W] |
1 |
0.1% |
| Tyr[Y] |
32 |
3.6% |
| Val[V] |
40 |
4.6% |
| Total Length |
879 |
|
| Instability index (II) |
48.69 |
| This classifies the protein as unstable |
|
| Carbon |
4284 |
| Hydrogen |
6673 |
| Nitrogen |
1221 |
| Oxygen |
1380 |
| Sulfur |
22 |
| Total number of atoms |
13580 |
| Formula = C4284H6673N1221O1380S22 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
53305 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
53180 M-1 cm-1 |
| assuming all Cys residues are reduced |
53180 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
53305 M-1 cm-1 |
|
|
| Codon Bias |
0.138 |
| Codon Adaptation Index |
0.178 |
| Frequency of Optimal Codons |
0.496 |
| Hydropathicity of Protein |
-0.747 |
| Aromaticity Score |
0.080 |
|
