Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
32 |
4.2% |
| Arg[R] |
38 |
5.0% |
| Asn[N] |
44 |
5.8% |
| Asp[D] |
57 |
7.5% |
| Cys[C] |
10 |
1.3% |
| Gln[Q] |
28 |
3.7% |
| Glu[E] |
54 |
7.1% |
| Gly[G] |
41 |
5.4% |
| His[H] |
9 |
1.2% |
| Ile[I] |
60 |
7.9% |
| Leu[L] |
91 |
12.0% |
| Lys[K] |
57 |
7.5% |
| Met[M] |
11 |
1.5% |
| Phe[F] |
22 |
2.9% |
| Pro[P] |
34 |
4.5% |
| Ser[S] |
59 |
7.8% |
| Thr[T] |
43 |
5.7% |
| Trp[W] |
1 |
0.1% |
| Tyr[Y] |
22 |
2.9% |
| Val[V] |
44 |
5.8% |
| Total Length |
757 |
|
| Instability index (II) |
42.58 |
| This classifies the protein as unstable |
|
| Carbon |
3753 |
| Hydrogen |
6085 |
| Nitrogen |
1019 |
| Oxygen |
1176 |
| Sulfur |
21 |
| Total number of atoms |
12054 |
| Formula = C3753H6085N1019O1176S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
38905 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
38280 M-1 cm-1 |
| assuming all Cys residues are reduced |
38280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
38905 M-1 cm-1 |
|
|
| Codon Bias |
0.129 |
| Codon Adaptation Index |
0.171 |
| Frequency of Optimal Codons |
0.483 |
| Hydropathicity of Protein |
-0.363 |
| Aromaticity Score |
0.059 |
|
