DAL5/YJR152W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 49 9.0%
Arg[R] 18 3.3%
Asn[N] 25 4.6%
Asp[D] 18 3.3%
Cys[C] 8 1.5%
Gln[Q] 13 2.4%
Glu[E] 27 5.0%
Gly[G] 29 5.3%
His[H] 6 1.1%
Ile[I] 47 8.7%
Leu[L] 54 9.9%
Lys[K] 34 6.3%
Met[M] 18 3.3%
Phe[F] 31 5.7%
Pro[P] 19 3.5%
Ser[S] 45 8.3%
Thr[T] 28 5.2%
Trp[W] 12 2.2%
Tyr[Y] 23 4.2%
Val[V] 39 7.2%
Total Length 543

Instability index
Instability index (II) 37.57
This classifies the protein as stable

Atomic composition
Carbon 2800
Hydrogen 4357
Nitrogen 693
Oxygen 768
Sulfur 26
Total number of atoms 8644
Formula = C2800H4357N693O768S26

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 100770 M-1 cm-1
assuming NO Cys residues appear as half cystines 100270 M-1 cm-1
assuming all Cys residues are reduced 100270 M-1 cm-1
assuming all pairs of Cys residues form cystines 100770 M-1 cm-1

Aliphatic index
Aliphatic index 102.39

Coding Region Translation Calculations
Codon Bias 0.143
Codon Adaptation Index 0.158
Frequency of Optimal Codons 0.491
Hydropathicity of Protein 0.273
Aromaticity Score 0.122