Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
66 |
4.0% |
| Arg[R] |
70 |
4.2% |
| Asn[N] |
106 |
6.4% |
| Asp[D] |
107 |
6.5% |
| Cys[C] |
19 |
1.2% |
| Gln[Q] |
68 |
4.1% |
| Glu[E] |
119 |
7.2% |
| Gly[G] |
54 |
3.3% |
| His[H] |
38 |
2.3% |
| Ile[I] |
119 |
7.2% |
| Leu[L] |
201 |
12.2% |
| Lys[K] |
128 |
7.8% |
| Met[M] |
28 |
1.7% |
| Phe[F] |
89 |
5.4% |
| Pro[P] |
47 |
2.9% |
| Ser[S] |
148 |
9.0% |
| Thr[T] |
76 |
4.6% |
| Trp[W] |
20 |
1.2% |
| Tyr[Y] |
73 |
4.4% |
| Val[V] |
72 |
4.4% |
| Total Length |
1648 |
|
| Instability index (II) |
45.22 |
| This classifies the protein as unstable |
|
| Carbon |
8647 |
| Hydrogen |
13458 |
| Nitrogen |
2256 |
| Oxygen |
2572 |
| Sulfur |
47 |
| Total number of atoms |
26980 |
| Formula = C8647H13458N2256O2572S47 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
219895 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
218770 M-1 cm-1 |
| assuming all Cys residues are reduced |
218770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
219895 M-1 cm-1 |
|
|
| Codon Bias |
0.040 |
| Codon Adaptation Index |
0.140 |
| Frequency of Optimal Codons |
0.438 |
| Hydropathicity of Protein |
-0.392 |
| Aromaticity Score |
0.110 |
|
