Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
88 |
6.1% |
| Arg[R] |
52 |
3.6% |
| Asn[N] |
82 |
5.7% |
| Asp[D] |
97 |
6.7% |
| Cys[C] |
7 |
0.5% |
| Gln[Q] |
56 |
3.9% |
| Glu[E] |
97 |
6.7% |
| Gly[G] |
89 |
6.2% |
| His[H] |
28 |
1.9% |
| Ile[I] |
81 |
5.6% |
| Leu[L] |
162 |
11.2% |
| Lys[K] |
102 |
7.1% |
| Met[M] |
16 |
1.1% |
| Phe[F] |
63 |
4.4% |
| Pro[P] |
66 |
4.6% |
| Ser[S] |
118 |
8.2% |
| Thr[T] |
87 |
6.0% |
| Trp[W] |
15 |
1.0% |
| Tyr[Y] |
51 |
3.5% |
| Val[V] |
85 |
5.9% |
| Total Length |
1442 |
|
| Instability index (II) |
35.74 |
| This classifies the protein as stable |
|
| Carbon |
7222 |
| Hydrogen |
11311 |
| Nitrogen |
1909 |
| Oxygen |
2225 |
| Sulfur |
23 |
| Total number of atoms |
22690 |
| Formula = C7222H11311N1909O2225S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
158865 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
158490 M-1 cm-1 |
| assuming all Cys residues are reduced |
158490 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
158865 M-1 cm-1 |
|
|
| Codon Bias |
0.251 |
| Codon Adaptation Index |
0.247 |
| Frequency of Optimal Codons |
0.552 |
| Hydropathicity of Protein |
-0.374 |
| Aromaticity Score |
0.089 |
|
