Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
54 |
10.6% |
| Arg[R] |
25 |
4.9% |
| Asn[N] |
11 |
2.2% |
| Asp[D] |
25 |
4.9% |
| Cys[C] |
1 |
0.2% |
| Gln[Q] |
20 |
3.9% |
| Glu[E] |
38 |
7.4% |
| Gly[G] |
46 |
9.0% |
| His[H] |
7 |
1.4% |
| Ile[I] |
34 |
6.7% |
| Leu[L] |
48 |
9.4% |
| Lys[K] |
29 |
5.7% |
| Met[M] |
8 |
1.6% |
| Phe[F] |
18 |
3.5% |
| Pro[P] |
25 |
4.9% |
| Ser[S] |
28 |
5.5% |
| Thr[T] |
37 |
7.2% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
11 |
2.2% |
| Val[V] |
45 |
8.8% |
| Total Length |
511 |
|
| Instability index (II) |
31.91 |
| This classifies the protein as stable |
|
| Carbon |
2443 |
| Hydrogen |
3953 |
| Nitrogen |
661 |
| Oxygen |
745 |
| Sulfur |
9 |
| Total number of atoms |
7811 |
| Formula = C2443H3953N661O745S9 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
21890 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
21890 M-1 cm-1 |
| assuming all Cys residues are reduced |
21890 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
21890 M-1 cm-1 |
|
|
| Codon Bias |
0.473 |
| Codon Adaptation Index |
0.425 |
| Frequency of Optimal Codons |
0.679 |
| Hydropathicity of Protein |
-0.018 |
| Aromaticity Score |
0.059 |
|
